The purpose of this study was to investigate attitudes toward non-traditional masculine/feminine traits and homosexuality among junior high school students in Taiwan. The sample consisted of 813 (398 males and 415 females) junior high school students. Four research instruments used in this study were: Attitudes toward Feminine Males (AFM), Attitudes toward Masculine Females (AMF), Attitudes toward Gay Peers (AGP), and Attitudes toward Lesbian Peers (ALP). The alpha coefficients were .92, .94. , .91 and .88 for AFM, AMF, AGP, and ALP, respectively. Results are indicated as follows: Students with different backgrounds report significant attitudes in AFM, AMF and AGP. Female students are more friendly than males, 9th graders are more friendly than 8th graders ,and students who have gay and lesbian friends have more positive attitudes than those who don’t have gay and lesbian friends. Students with different backgrounds express significantly different scores in ALP. Female students are more friendly than males, and students who have gay and lesbian friends have more positive attitudes than do their courterparts. There is a moderate positive correlation between AFM and AGP. There is a low positive correlation between AMF and ALP. The partial mediation of AFM is significant between students’ backgrounds and AGP. The partial mediation of AMF is significant between students’ backgrounds and ALP. Finally, suggestions for education administrators, junior high school teachers, and students are presented.